 |
| In contrast to the often-asked krill vs. fish oil question, the issue of PUFA vs. SFA seems to be resolved. At least if we focus on replacing SFA with N-6-PUFA well-controlled RCTs don't show a benefit. |
Learn more about various topics at the SuppVersity
Apples a day + Lot's of Water = HEALTH
Healthy Fats for Pregnancy Diets - Reviewed
Red Meat a 'No-Go' Only for the Obese?
White Bread Not Bad W/ Right Microbiome
Low Food Quality, Not High Sugar Drives Obesity
Avoid Protein Oxidation, Stay Healthy
- Fish and krill oil - similar, but significantly different - Years ago, in the early days of the SuppVersity I wrote about the "phospholipid advantage" of krill vs. fish oil. While the latter comes in conventional triglycerides, a large percentage of the omega-3 fatty acids in krill oil are already in the preferable phospholipid form that can be readily incorporated into our cells. That's a theoretical advantage, of which a 2013 review by Schuchardt et al. does yet say that it is "often claimed", but eventually hasn't been demonstrated "conclusively".
Nevertheless, studies such as Ramprasath et al (2013), which was published after Schuchardt et al. stopped collecting data for their previously cited review seem to confirm that "krill oil could be more effective than fish oil in increasing n-3 PUFA, reducing n-6:n-3 PUFA ratio, and improving the omega-3 index" (Ramprasath 2013).![]()
Figure 1: The 4-week double blinded, randomized, placebo-controlled, crossover trial by Ramprasaht et al (2013) is probably the best comparison of the bioavailability of fish and krill oil and it shows significant advantages of krill oil, especially in terms of the health-relevant incorporation of N-3 PUFA in red blood cells.
Eventually, bioavailability is yet not everything that counts and that's why the results of a recent study from the Victoria University in Australia (Sung 2017) are so intriguing. In their RCT, the researchers gave 11 healthy women (18-45 years) either 1.3 g of total long-chain n-3 PUFA from krill oil (KO) or 1.4 g from fish oil (FO) for 30 days. The detailed analysis of the scientists' mass-spectrometer data revealed ...- a total of 11 EPA and 40 DHA containing molecular species which were significantly increased in both KO and FO supplementation with no significant differences between groups, apart from phosphatidylethanolamine (16:0-22:6, p < 0.02), and
- a differential effect of FO and KO on 6 out of the 51 arachidonic acid (AA) molecular species, with KO significantly increasing two and decreased two of these AA species, whereas FO significantly increased one and decreased seven of the AA species.
What's the verdict on Fish vs. Krill oil, then? Unfortunately, the biological significance of these changes is almost as elusive as the complex effects of the other changes in arachidonic acid metabolism, of which we have almost no idea if they can health-relevant effects and would warrant switching from cheap fish to significantly more expensive krill oil.
- Latest meta-analysis exposes shortcomings of previous papers suggesting that replacing saturated fat with omega-6 PUFAs would be heart healthy -- Unfortunately, the recommendation to replace saturated fatty acids (SFA) with mostly n-6 polyunsaturated fatty acids (PUFA) to reduce the risk of coronary heart disease (CHD) is still a cornerstone of conventional dietary advice - advice, of which its advocates claim that it was based on 'many clinical trials and meta-analysis'. This allegedly 'convincing' evidence has yet been questioned by recent meta-analyses of observational studies and clinical trials (Siri-Tarino 2010; Ramsden 2010; Chowdhury 2014; Harcombe 2015; Ramsden 2016).
As Steven Hamley points out in a recently published meta-analysis, "earlier meta-analyses did not sufficiently account for major confounding variables that were present in some of those trials" (Hamley 2017). That's a bummer, a major bummer as Hamley's re-analysis of the data shows, because after removing ‘inadequately controlled’ trials, the previously significant advantage in terms of CHD events (-20%, p = 0.03) disappeared.
The already non-significant improvements in major CHD events, CHD mortality and total mortality were even reversed - note: since the meta-analysis of adequately controlled studies yielded only non-significant results, that's not grist to the mills of the saturated fat advocates, though.![]()
Figure 2: Even when Hamley included all studies (also the non-adequately controlled study), only the risk for total CHD events was significantly reduced. When Hamley analyzed only the adequately controlled study, the risk reductions were not just non-significant, they simply didn't exist (data from Hamley 2017). What's the verdict on replacing saturated fat with omega-6 fats, then? If only those trials that controlled adequately for the various dietary and behavioral confounders, the previously reported benefits of replacing SFA with n-6 PUFAs, which are at the basis of contemporary dietary advice, disappear.
- Low-FODMAP diet may help athletes prevent GI distress -- You've read me write about the potential usefulness of low-FODMAP diets in IBS before. That avoiding oligosaccharide, disaccharide, monosaccharide, and polyols, of which some research shows that they can fuel healthy gut bacteria, should be beneficial for athletes, on the other hand, is news; news that's based on a soon-to-be-published study by Lis et al (2017).
Inspired by the promising trials in IBS sufferers, a team of Canadian researchers tried to figure out if avoiding FODMAPS would produce similar reductions in GI distress parameters in runners with a history of non-clinical exercise-associated GI symptoms as it did in recent IBS trials. To this ends, Li et al. recruited eleven recreationally competitive runners (5 males, 6 females; 5km personal best 23:00+/-4:02 min: sec), who were allocated to a randomized 6-day low (LFOD) or high FODMAP (HFOD) diet in a controlled, single-blinded cross-over study.
In each period participants completed two strenuous running sessions consisting of 5x1000m and a 7km threshold run. GI symptoms (during-exercise and daily) and the Daily Analysis of Life Demand for Athletes (DALDA) questionnaires, which monitor the state of well being and mood state, were completed. To measure the effects, the scientists calculated the area under the curve (AUC) for daily GI symptoms across each dietary period and analyzed it using multilevel mixed-effects linear regression to compare the effects of the two isocaloric diets (note: while the LFOD diet was lower in protein and higher in fat, the absolute differences were 5 and 7g and can thus be neglected despite being statistically significant; p = 0.03 and p = 0.003, respectively, what may be more important, though, is the lower GI of LFOD).![]()
Figure 3: Schematic showing participant selection and study design. LFOD=Low FODMAP diet, HFOD=High FODMAP diet, GI=gastrointestinal, DALDA=Daily Analysis of Life Demands for Athletes (Lis 2017).
As you can see in Figure 4, Li et al. recorded a significantly smaller AUC for daily GI symptoms over the 6-day period during the LFOD compared to HFOD (mean difference -13.4, 95% CI [-22, -4.60], P=0.003).![]()
Figure 4: The AUC [~sum of] for daily GI symptoms is the only marker that improved significantly (Lis 2017)
The daily GI symptoms that were significantly lower during LFOD were flatulence (P<0.001), urge to defecate (P=0.04), loose stool (P=0.03) and diarrhea (P=0.004). No significant differences in during exercise symptoms or DALDA responses were observed between diets (p>0.05), however.What's the verdict on low-FODMAP diets for athletes, then? In view of the fact that these preliminary findings suggest that short-term FODMAP reduction may be a beneficial intervention to minimize daily GI symptoms in runners with exercise-related GI distress, I would love to see follow-ups with (a) longer wash-out periods (the single day used in the study at hand seems to be very short considering the fact that FODMAPs may take 3 days to pass the digestive tract (de Oliveira 2017)), (b) longer study periods , in order to measure potential effects on the microbiome and chronic inflammation, and (c) different exercise conditions, including those ultra-intense/long endurance races that are most prone to trigger GI distress... ah, and did I say that I would like to see someone ensure that removing foods high in FODMAPS doesn't reduce athletic performance? Well, now I did ;-)
 |
| Remember? Fish and krill oil also have different epigenetic effect | more. |
- Chowdhury, Rajiv, et al. "Association of dietary, circulating, and supplement fatty acids with coronary riska systematic review and meta-analysis." Annals of internal medicine 160.6 (2014): 398-406.
- de Oliveira, Erick P. "Runner's diarrhea: what is it, what causes it, and how can it be prevented?." Current opinion in gastroenterology 33.1 (2017): 41-46.
- Harcombe, Zoë, et al. "Evidence from randomised controlled trials did not support the introduction of dietary fat guidelines in 1977 and 1983: a systematic review and meta-analysis." Open heart 2.1 (2015): e000196.
- Ramprasath, Vanu R., et al. "Enhanced increase of omega-3 index in healthy individuals with response to 4-week n-3 fatty acid supplementation from krill oil versus fish oil." Lipids in health and disease 12.1 (2013): 178.
- Ramsden, Christopher E., et al. "n-6 fatty acid-specific and mixed polyunsaturate dietary interventions have different effects on CHD risk: a meta-analysis of randomised controlled trials." British Journal of Nutrition 104.11 (2010): 1586-1600.
- Ramsden, Christopher E., et al. "Re-evaluation of the traditional diet-heart hypothesis: analysis of recovered data from Minnesota Coronary Experiment (1968-73)." bmj 353 (2016): i1246.
- Schuchardt, Jan Philipp, et al. "Incorporation of EPA and DHA into plasma phospholipids in response to different omega-3 fatty acid formulations-a comparative bioavailability study of fish oil vs. krill oil." Lipids in health and disease 10.1 (2011): 145.
- Schuchardt, Jan Philipp, and Andreas Hahn. "Bioavailability of long-chain omega-3 fatty acids." Prostaglandins, Leukotrienes and Essential Fatty Acids (PLEFA) 89.1 (2013): 1-8.
- Siri-Tarino, P. "Meta-analysis of prospective cohort studies evaluating the association of saturated fat with cardiovascular disease. Retrieved June 20, 2015." (2010).
- Sung, H. H., et al. "Lipidomic profiles after a 30-day krill oil supplementation compared with fish oil in healthy women." Journal of Nutrition & Intermediary Metabolism 8 (2017): 83-84.